Table 4.
Affinity of ABC solute-binding proteins for maltooligosaccharides and monosaccharides
| EUR_01830 | EUR_31480 | EUR_01240 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Ka (μM−1) | n | Kd (nM) | Ka (μM−1) | n | Kd (nM) | Ka (μM−1) | n | Kd (nM) | |
| Glucose | -- | -- | -- | NB | NB | NB | 0.64 ± 0.13 | 0.56 ± 0.035 | 1900 ± 460 |
| Galactose | -- | -- | -- | -- | -- | -- | 2.41 ± 0.12 | 0.56 ± 0.045 | 415 ± 20.2 |
| Maltose | NB | 1.79 ± 0.021 | 0.88 ± 0.003 | 563 ± 66.4 | NB | ||||
| Isomaltose | NB | NB | -- | ||||||
| Panose | NB | NB | -- | ||||||
| Maltotriose | 1.82 ± 0.05 | 0.88 ± 0.06 | 550 ± 14 | 2.82 ± 0.0035 | 0.87 ± 0.024 | 355 ± 0.4 | -- | ||
| Maltotetraose | 9.10 ± 1.15 | 1.12 ± 0.08 | 112 ± 19.8 | 0.60 ±0.072 | 0.96 ± 0.063 | 1670 ± 199 | -- | ||
| Maltopentaose | 5.26 ± 0.85 | 0.77 ± 0.10 | 194 ± 34.8 | NB | -- | ||||
| Maltohexaose | 6.27 ± 0.03 | 0.80 ± 0.10 | 160 ± 6 | NB | -- | ||||
| Maltoheptaose | 3.47 ± 0.07 | 0.82 ± 0.01 | 288 ± 5.9 | NB | -- | ||||
| Acarbose | 3.33 ± 0.24 | 0.81 ± 0.04 | 302 ± 21.8 | NB | -- | ||||
| GM | 0.11 ± 0.008 | 0.87 ± 0.08 | 9080 ± 618 | NB | -- | ||||
Average results of 2–3 titrations reported.
NB = No observable binding
-- = Experiment was not performed.