Subcellular localization of TSP1, TSP1CT6, TSP3 and TSP3CT6 in absence of host
cells. Cells expressing C-terminal HA-tagged versions of TSP3 (TVAG_280860),
TSP3CT6 (chimera with the C-terminal tail of TSP3 replaced by the C-terminal
tail of TvTSP6), TSP1 (TVAG_019180) and TSP1CT6 (chimera with the C-terminal
tail of TSP1 replaced by the C-terminal tail of TvTSP6) were stained for
immunofluorescence microscopy using an anti-HA antibody. The nucleus (blue) was
also stained with DAPI. Scale bar, 10 μm. Note the intensity of flagella
signal on the chimeric proteins TSP1CT6 and TSP3CT6 relative to wild-type TSP1
and TSP3 proteins.