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. Author manuscript; available in PMC: 2015 May 19.
Published in final edited form as: Cell Microbiol. 2012 Aug 29;14(12):1797–1807. doi: 10.1111/cmi.12003

Fig. 6.

Fig. 6

Subcellular localization of the TvTSP1CT6 protein in parasites bound to VECs.

A. Parasites transfected with TvTSP1CT6 containing a C-terminal HA tag were bound to VECs for 0.5, 1, 3 and 6 h and the protein was localized using an anti-HA antibody. Note the change in localization from plasma membrane and intracellular vesicles to flagellar localization at 3 and 6 h post host cell exposure. Flagella (red arrows) and the main body of the cell (white arrows) were indicated. Scale bar, 10 μm.

B. Percentage of cells transfected with TvTSP1 and TvTSP1CT6 with the respective protein detected on the flagella only (grey columns) or flagella and plasma membrane (black columns) during exposure to VECs. One hundred parasites were counted in triplicate in four independent experiments.