Subcellular localization of the TvTSP1CT6 protein in parasites bound to VECs.
A. Parasites transfected with TvTSP1CT6 containing a C-terminal HA tag were bound
to VECs for 0.5, 1, 3 and 6 h and the protein was localized using an anti-HA
antibody. Note the change in localization from plasma membrane and intracellular
vesicles to flagellar localization at 3 and 6 h post host cell exposure.
Flagella (red arrows) and the main body of the cell (white arrows) were
indicated. Scale bar, 10 μm.
B. Percentage of cells transfected with TvTSP1 and TvTSP1CT6 with the respective
protein detected on the flagella only (grey columns) or flagella and plasma
membrane (black columns) during exposure to VECs. One hundred parasites were
counted in triplicate in four independent experiments.