. Author manuscript; available in PMC: 2015 May 19.

Published in final edited form as: Mol Microbiol. 2013 Nov 20;91(1):198–208. doi: 10.1111/mmi.12455

Table 3.

Kinetic parameters of recombinant FR1, FR5, and FR6. ND…not determined.

	FR1	FR5	FR6
Km FMN [μM]	10 ± 1.4	186.9 ± 30.3	160 ± 23.9
Km FAD [μM]	16.5 ± 2.5	267.4 ± 21.6	192.1 ± 12.2
Km riboflavin/(+ 400 nM FMN) [μM]	148.6 ± 29.4 (22.5 ± 4.6)	94.4 ± 11.5	97.5 ± 28.8
Km NADPH [μM]	89.4 ± 12.7	ND	ND
Vmax FMN [μmol sec^-1 mg^-1]	236. 4 ± 9,1	185.2 ± 12.2	170.8 ± 11.3
Vmax FAD [μmol sec^-1 mg^-1]	73.5 ± 3.8	163.8 ± 7	154.4 ± 4.6
Vmax riboflavin/(+ 400 nM FMN) [μmol sec^-1 mg^-1]	136.3 ± 14.6 (129.3 ± 9.7)	144.9 ± 8.2	158.7 ± 22