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. Author manuscript; available in PMC: 2015 May 19.
Published in final edited form as: Mol Microbiol. 2013 Nov 20;91(1):198–208. doi: 10.1111/mmi.12455

Table 3.

Kinetic parameters of recombinant FR1, FR5, and FR6. ND…not determined.

FR1 FR5 FR6
Km FMN [μM] 10 ± 1.4 186.9 ± 30.3 160 ± 23.9
Km FAD [μM] 16.5 ± 2.5 267.4 ± 21.6 192.1 ± 12.2
Km riboflavin/(+ 400 nM FMN)
[μM]
148.6 ± 29.4
(22.5 ± 4.6)
94.4 ± 11.5 97.5 ± 28.8
Km NADPH [μM] 89.4 ± 12.7 ND ND
Vmax FMN [μmol sec-1 mg-1] 236. 4 ± 9,1 185.2 ± 12.2 170.8 ± 11.3
Vmax FAD [μmol sec-1 mg-1] 73.5 ± 3.8 163.8 ± 7 154.4 ± 4.6
Vmax riboflavin/(+ 400 nM FMN)
[μmol sec-1 mg-1]
136.3 ± 14.6
(129.3 ± 9.7)
144.9 ± 8.2 158.7 ± 22