Sham or OVX surgeries were performed on 4-month-old EP1−/− and WT mice. Trabecular bone regions in the metaphyseal femur [A] and the vertebral body [D] were examined. [B & E] Bone volume/total volume; [C & F] trabecular number, trabecular spacing, and trabecular thickness quantification of the femur and L4 vertebrae, respectively. N = 5 per group. Statistical analysis was performed using 2-way ANOVA followed by Dunnett’s test. (*) = p < 0.05 v.s. WT sham, ($) = p<0.05 vs KO Sham, (#) = p < 0.05 v.s. WT OVX.