Skip to main content
. 2015 May 19;10(5):e0127288. doi: 10.1371/journal.pone.0127288

Fig 3. Metal binding affinities (Kd) of DddW.

Fig 3

Titration of apo-DddW with increasing concentrations of the metal ions Fe(NH4)2(SO4)2 (added anaerobically), FeCl3, CoCl2, MnCl2, CuCl2 or NiCl2 was done. The binding was monitored by saturation of the fluorescence intensities. The concentration of enzyme used is as follows: (A) 0.5 μM apo-DddW, (B)-(F) 2 μM apo-DddW. The Kd values were: (A) Fe(II), 4.7 ± 0.0 nM; (B) Fe(III), 89.3 ± 4.3 μM; (C) Mn(II), 32.7 ± 5.0 μM; (D) Co(II), 2.5 ± 0.4 μM; (E) Ni(II), 1.0 ± 0.1 μM; (F) Cu(II), 1.9 ± 0.2 μM.