Fig 5. Effects of various transition metal ions on the DMSP lyase activity of DddW.
All metal ions were added so that they were at least 90% bound (the calculations were based on the Kd of individual metal ions). Assays were performed anaerobically at pH 8.0 and room temperature. The activity of Apo-DddW without metal ions was used as a reference (100%). (A) Determination of the initial velocity (Vi) of DddW catalyzed reaction in presence of various transition metal ions: Mn(II), Fe(II), Fe(III), Co(II), Ni(II), Cu(II), and Zn(II). For clarity, plots of Fe(III), Zn (II), apo-DddW, and buffer control are indicated by ‘*’. (B) Effect of metal ions on the initial velocity (Vi) of DddW catalyzed lyase reaction.