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. Author manuscript; available in PMC: 2015 May 19.
Published in final edited form as: J Control Release. 2013 Jun 2;170(2):219–225. doi: 10.1016/j.jconrel.2013.05.023

Fig. 2.

Fig. 2

Site-specific incorporation of pEthF into the mDHFR-38Am and CuAAC-mediated coumarin-labeling. (A) MALDI-TOF analysis of trypsin-digested mDHFR-WT and mDHFR-pEthF. Peptide F38 (top) of the mDHFR-WT and Peptide Z38 of the mDHFR-pEthF (bottom). (B) Protein gel images of the fluorogenic dye-treated mDHFR-pEthF (pEthF) and mDHFR-WT (WT). The gel was subjected to UV (390 nm) irradiation to excite the fluorophore (fluorescence panel), and then stained with Coomassie brilliant blue (Coomassie panel) to visualize proteins.