Table 1.
Nucleotide sequence variations of HPV-16 E6 and E7 genes, predicted amino-acid changes ant lineage classification in Southwest Congolese women
| E6 nucleotide position | E7 nucleotide position | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amino acid changes | R10G | R101/R10T | L12I | L12S | P13G | Q14D | Q14H | E29Q | C51F | A6IG | D64E | H78Y | D2IN | N29S | ||||||||||
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 4 | 6 | 6 | 7 | 7 | ||||
| 0 | 3 | 3 | 3 | 3 | 4 | 4 | 4 | 4 | 8 | 5 | 8 | 8 | 8 | 9 | 3 | 0 | 2 | 4 | 8 | 9 | ||||
| 9 | 1 | 2 | 7 | 8 | 1 | 2 | 3 | 5 | 8 | 5 | 5 | 6 | 9 | 5 | 5 | 3 | 2 | 7 | 9 | 5 | ||||
| HPV16 Ref (NC_001526) | T | A | G | T | T | C | A | C | G | G | G | C | T | A | T | C | A | G | A | T | T | |||
| Variant Lineages | Variant Sub-lineages | Novel nomenclature | Prevalence | |||||||||||||||||||||
| No. (%) | ||||||||||||||||||||||||
| Af-1 | Af-la | B1 | 4 (30.7) | C | g | G | T | C | a | g | T | c | g | |||||||||||
| Af-Ib/G295 | B2 | 1 (7.7) | C | G | T | a | g | G | T | c | g | |||||||||||||
| Af-Ib/G131 | B2 | 2 (15.4) | G | C | G | T | a | g | T | c | g | |||||||||||||
| Af-2 | Af-2a/r | C/C1 | 3 (23.0) | c | T | G | T | a | g | T | g | A* | c | g | ||||||||||
| Af-2a/C109/C138 | 1 (7.7) | c | T | C | G | T | a | g | G | T | g | G | c | g | ||||||||||
| Af-2a/C109/A137 | 1 (7.7) | c | T | A | G | G | T | a | g | T | g | G | c | g | ||||||||||
| Af-2a/C109/G285 | 1 (7.7) | c | T | G | T | G | a | g | T | g | G | c | g | |||||||||||
| Mutation prevalence (%) | 46.1 | 15.4 | 53.8/46.1 | 7.7 | 7.7 | 7.7 | 7.7 | 100 | 100 | 30.7 | 15.4 | 7.7 | 100 | 100 | 7.7 | 100 | 46.1 | 23.0 | 23.0 | 100 | 100 | |||
Capital letters indicate variants with an amino acid change, Lower-case letters indicate silent mutations, M, missense; S, silence; The asterisk (*) indicates mutations never described.