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. Author manuscript; available in PMC: 2015 May 19.
Published in final edited form as: Cytokine. 2011 Apr 27;55(1):110–115. doi: 10.1016/j.cyto.2011.03.003

Fig. 3.

Fig. 3

A small EF induces significant increase of VEGF mRNA in endothelial cells. Two major VEGF isoforms, VEGF165 and 121, were amplified. (A) Semi-quantitative RT-PCR with β-actin as internal control; (B and C) quantification of mRNA levels. Isolated total RNA from control and EF-stimulated HUVEC were analyzed by semi-quantitative RTPCR: histogram indicating the mRNA expression of VEGF165 and 121 normalized to β-actin (expressed as fold change relative to the 0 h control). VEGF mRNA transcription increased significantly at all the time points 4–24 h (up to 33% increase compared with control). The error bars represent the SE (*p < 0.05).