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. 2015 May 19;10(5):e0125960. doi: 10.1371/journal.pone.0125960

Fig 7. Expression of ChPMA1 and ChPMA2.

Fig 7

(A) Quantitative RT-PCR analysis of transcript abundance for ChPMA1 and ChPMA2 in conidia, in vitro appressoria (AP), mycelium grown 48 h or 72 h in liquid modified Mathur’s medium (M) and during infection of A. thaliana after three or four days (3 dpi, 4 dpi). Data was normalized against alpha-tubulin (CH063_01222) expression and plotted relative to alpha-tubulin (average from three biological replicates). (B) Normalized ChPMA2 expression plotted relative to ChPMA2 level in conidia. (C, D) Confocal images of C. higginsianum transformed with the promoter fusions Ppma2-mCherry (pCK3880) or Ppma1-GFP (pCK3973) after 0 to 32 h of incubation on coated ibidi μ-dishes (C) or after one and three days of spray infection of A. thaliana (D). The images are depicted as overlays of bright-field channel with mCherry or GFP-channel (C) or with an additional chloroplast autofluorescence channel in blue (D). Scale bar = 10 μm.