Upper panel: Representative western blots for AT2R with loading control β-actin. Bar graphs represent the ratios of densities of AT2R and β-actin protein bands. Results are means ± SEM; * -significantly different from Ovi on ND, $ - significantly different from Ovx on ND, @ - significantly different from Ovx on HFD. Data were analyzed using one-way ANOVA with Newman-keuls post hoc test and Student's t test (p<0.05); N=3-5 in each group. (ND - Normal Diet, HFD -High-Fat Diet, HFD-C21 - High-Fat Diet on C21, HFD-E - High-Fat Diet on E2, HFD-E+C21 -High-Fat Diet on E2+C21).