. Author manuscript; available in PMC: 2016 May 18.

Published in final edited form as: Chem Res Toxicol. 2015 Apr 10;28(5):1045–1059. doi: 10.1021/acs.chemrestox.5b00075

Table 1.

A list of identified peptide adducts from N-sulfooxy-PhIP and N-sulfooxy-[²H₅]-PhIP, N-acetoxy-PhIP and N-acetoxy-[²H₅]-PhIP, or HONH-PhIP and HONH-[²H₅]-PhIP modified albumin using mass tag triggered data dependent scanning method.

Proteolytic Enzymes	Peptides Adducts	Modified sites
Trypsin/ chymotrypsin	LQQC^*[SOPhIP]PFEDHVK	Cys³⁴
	LQQC^*[SO₂PhIP]PFEDHVK	Cys³⁴
	LQQC^*[SOPhIP]PF	Cys³⁴
Pronase E/ leucine aminopeptidas/ prolidase	C^[SO₂PhIP]	Cys³⁴
	C^*[SO₂PhIP]PF	Cys³⁴
	W^[PhIP]	Trp²¹⁴
	H^[PhIP]	His

The His residues of SA that reacted with N-oxidized PhIP are uncertain. There are 16 His residues in SA and the His containing PhIP adduct(s) was identified as a mono amino acid adduct following pronase digestion.