Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Splice product detection by RT-PCR. Total RNA was extracted from HCV infected cells at 48 h and the spliced products were detected by RT-PCR. (A) Top: introns cells, + reverse transcriptase (RT); bottom: -RT; (B) mock and uninfected intron cells; (C) ΔIntrons containing only guide sequences. Arrow indicates splice product; (D) DNA sequencing of the splice junction. Bordered indicates Uracil target.
