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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 13;1849(6):637–652. doi: 10.1016/j.bbagrm.2015.03.007

Figure 6. p50 overexpression blocks recruitment of p65 to the CCL2 and CCL20, but not COX2, gene promoters.

Figure 6

A–H. 832/13 cells were transduced with adenoviruses overexpressing βGAL or p50 (NF-κB1). 24 h post-transduction cells were untreated (NT) or stimulated with 1 ng/mL IL-1β for 15 mins. ChIP assays were performed to determine relative occupancy of p65 (A, C, E, G) and p50 (B, D, F, H) on the CCL20 proximal (A, B) and distal promoters (C, D), and on the CCL2 (E, F) and COX2 (G, H) promoters. **, P < 0.01 vs. βGAL (IL-1β), *, P < 0.05 vs. βGAL (IL-1β), #P < 0.1 vs. βGAL (IL-1β). ChIP data are shown as means ± SEM from 3 individual experiments.