Figure 7. Differential co-activator recruitment in response to IL-1β.
A, C, E. 832/13 cells were untreated (NT) or stimulated with 1 ng/mL IL-1β for 15 mins. ChIP assays were used to determine relative occupancy of p65, CARM1, p300 and CBP on the CCL20 proximal promoter (A), and on the CCL2 (C) and COX2 (E) promoters. ***, P < 0.001 vs. NT, **, P < 0.01 vs. NT, *, P < 0.05 vs. NT. B, D, F. 832/13 cells were untreated (NT) or stimulated with 1 ng/mL IL-1β for 15, 30 or 60 mins. ChIP assay of histone H3 dimethylated on Arginine 17 (H3R17me2) is shown at the CCL20 proximal promoter (B), and on the CCL2 (D) and COX2 (F) promoters. *, P < 0.05 vs. NT, #P < 0.1 vs. NT. Data are shown as means ± SEM from 3 individual experiments.