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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Virology. 2015 Mar 9;481:13–23. doi: 10.1016/j.virol.2015.02.008

Figure 6. pp71 associates with polysomes during HCMV infection.

Figure 6

(A) Lysate from infected HFFs (72 hpi) was resolved through a 10–50% linear sucrose gradient and the gradient was fractionated. Aliquots from each fraction were either TCA precipitated and analyzed by Western blot (top) or total RNA was extracted and separated on an agarose gel to visualize ribosomal RNA (bottom). The black bar denotes the location of polysome-containing fractions. (B) Seventy two hours after infection cells were lysed in buffer with or without added EDTA. The lysates were separated by sucrose gradient centrifugation as in A and aliquots of the polysomes-containing fractions were pooled, precipitated and analyzed by Western blots with antibodies specific to pp71, UL69 or rpS6. The data are representative of at least two independent experiments.