Fig 6. Induction of var2csa in HB3 specifically activates the locus on chromosome 12.

The nucleotide sequences of the two copies of var2csa found within the genome of HB3 are shown. Of note are the polymorphisms (surrounded by red boxes) that easily distinguish the gene found on chromosome 1 from that found on chromosome 12. In particular, two highly polymorphic regions are labeled. The location and sequence of PCR primers (red text) that amplify across these polymorphic regions are shown. Sequencing of this PCR product after induction of var2csa expression by chaetocin treatment or over-expression of PfSET2 dom-neg detected only the locus on chromosome 12.