Table 2. Primer sets used in real-time PCR assays specifically to amplify var2csa from HB3.
Primer set | Forward Primer | Reverse Primer |
---|---|---|
1 (73) | CCAAAATATAGCGAGCACA | CCTTCATCTTGCTCTTGTCG |
2 (75) | TAGTGAACCTATTTATATTCGT | TGCTTCATTTCCGATGTTTG |
3 (79) | TTTCTCAAGGATTGTGAGAGGTC | TTCATAGCTTCTAGCGCCTTTT |
The numbers in parentheses are the original primer set # from Salanti et al. [43].