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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Clin Genet. 2014 Dec 26;88(5):494–498. doi: 10.1111/cge.12541

Figure 2. Effect of the p.Gln232Argfs*3 mutation upon GRHPR activity and expression in vitro.

Figure 2

Lysates from CHO cells transfected with wild-type (Wild-type) or mutant c.694delC/p.Gln232Argfs*3 (c.694delC) GRHPR or from untransfected CHO cells (None) were assayed for enzymatic activity (A) and immunodetectable GRHPR (B). In A, enzymatic activity of the mutant (expressed as nmol glycolate produced/min/mg total cell protein) was not different from background activity in untransfected cells (None), whereas activity was detected in cells expressing wild-type GR (Wild-type), and in mouse liver homogenate (positive control; 3620 ± 730; not shown). In B, GRHPR and actin (loading control) immunoreactivity is shown for lysates prepared from untransfected CHO cells (None) and CHO cells transfected with wild-type (Wild-type) or mutant (c.694delC) cDNA. Mouse liver serves as an additional control. Data are mean ± SEM.