Overexpression of miR-126 modulates IGF-1/AKT/GSK-3β and ERK signaling in STS and IGF-1 treated neurons. Quantification of Western blots (a) shows that IRS-1 (b), AKT, pAKT, ERK, pERK, GSK-3β, and pGSK-3β (c) are downregulated in miR-126 overexpressing cells when compared to virus-treated controls. In addition, the pAKT/AKT, pERK/ERK and pGSK-3β/GSK-3β ratios are increased, and the pGSK-3β/GSK-3β ratios decreased in STS/IGF-1 treated and miR-126 transduced cells. Data are plotted as relative percent expression to untreated controls. *: p < 0.05 comparing treated to untreated condition. #: p < 0.05 comparing ratios for Syn.miR-126 to ratios of Syn.GFP controls.