Fig. 7.
Inhibition of miR-126 is neuroprotective. (a, b) Naive PC12 cells, or transduced cell lines that express a virus control or doxycycline (Dox)-inducible miR126 [28] were transfected with 70–100 nM scrambled (LNAsc) or miR-126 targeting LNAs (LNA126) and treated with 300 nM STS and 20 ng/ml IGF-1 (a), or 2 μM Aβ1-42 (b). LDH assays show that both IGF-1 and LNA126 improve cell survival in STS-untreated cells and are neuroprotective in STS-treated conditions (*: p < 0.05 comparing cell death relative to untreated LNAsc controls). Similarly, LNA126 improve survival of Aβ1-42-untreated cells and are neuroprotective in Aβ1-42-treated conditions. (*: p < 0.05 comparing LNAsc/Aβ1-42 + to LNAsc/Aβ1-42 −; #: p < 0.05 comparing LNA126/Aβ1-42− to LNAsc/Aβ1-42 −; §: p < 0.05 comparing LNA126/Aβ1-42+ to LNAsc/Aβ1-42+).