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(A) Substrate of dihydropyrimidinase, hydantoinase, imidase, allantoinase, and dihydroorotase. Kinetic analysis of dihydropyrimidinase was carried out using (B) dihydrouracil, (C) phthalimide, and (D) 5-propyl-hydantoin as a substrate. The maximal concentration of phthalimide was limited to 1 mM due to its poor solubility. Data points are an average of 2–3 determinations within 10% error.
