Table 1. Primers used for construction of plasmids.
Oligonucleotide | Primer |
---|---|
WT-NdeI-N | CGCGGCATATGTTTGATTTACTCCTGC |
WT-XhoI-C | TCGCACTCGAGAAAATCGAAGGCATGT |
H59A-N | GGCATCGACCCCGCTACCCACATGCAGTTGCCC |
H59A-C | CAACTGCATGTGGGTAGCGGGGTCGATGCCGCC |
H61A-N | GACCCCCATACCGCCATGCAGTTGCCCTTCATG |
H61A-C | GAAGGGCAACTGCATGGCGGTATGGGGGTCGAT |
H183A-N | GCGGTGCCGACGGTGGCCGCGGAGAACGGCGAA |
H183A-C | GCCGTTCTCCGCGGCCACCGTCGGCACCGCGCC |
H239A-N | CTGTACCTGGTAGCTATTTCCAGCCGCGAGGCG |
H239A-C | CTCGCGGCTGGAAATAGCTACCAGGTACAGCGG |
K150A-N | GTGAACAGCTTCGCGCACTTCATGGCCTACAAG |
K150A-C | GTAGGCCATGAAGTGCGCGAAGCTGTTCACCCC |
D316A-N | ACCACCGCCACCGCCCACTGCTGCTTCTGCGCC |
D316A-C | GCAGAAGCAGCAGTGGGCGGTGGCGGTGGTATG |
Y155A-N | CACTTCATGGCCGCCAAGAACGCCATCATGGCC |
Y155A-C | CATGATGGCGTTCTTGGCGGCCATGAAGTGCTT |
S289A-N | GGCTACGTGATGGCCCCGCCGTTCCGTCCCGTC |
S289A-C | GGGACGGAACGGCGGGGCCATCACGTAGCCGGC |
N337A-N | TTCAGCAAGATTCCCGCTGGCACGGCCGGCATC |
N337A-C | GCCGGCCGTGCCAGCGGGAATCTTGCTGAAGTC |
These plasmids were verified by DNA sequencing. Underlined nucleotides indicate the designated site for mutation or the restriction site.