Figure 2.

Identification of the stemness of rat hepatic stellate cells. A: The expression of all the stem cell markers (CD133, CD105, and nestin) was positive in HSC-T6 cells; B: After transfection of adenovirus AdHNF4α, the expression of HNF4α and Nanog was observed by co-focal immunofluorescent staining; original magnification × 400; C: The relative expression levels of HNF4α and Nanog were calculated by image density analysis with the Image-Pro Plus V6.0 (Media Cybernetics, Inc., Rockville, MD, United States) normalized with DAPI staining. HNF4α: Hepatocyte nuclear factor 4α; HSCs: Hepatic stellate cells.