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. 2015 May 19;10(5):e0125912. doi: 10.1371/journal.pone.0125912

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© 2015 Gebremedhn et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — The relative abundance of individual miRNAs is presented as the ratio of preovulatory dominant and subordinate follicles. The mean expression value of target miRNA was normalized against the expression of endogenous control U6 snRNA and 5s rRNAs. The normalized miRNA expression (2^-ΔCT) in preovulatory dominant follicle was divided by the normalized miRNA expression in subordinate follicle and Log₂ transformed. MiRNAs with Log₂ ratio > 0 are up-regulated in preovulatory dominant follicles and miRNAs with Log₂ ratio < 0 are down-regulated in preovulatory dominant follicles compared to the subordinate follicles counterparts. Statistical significance of each miRNA are represented by asterisks; *, p<0.05 and **, p<0.01.