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. 2015 May 19;10(5):e0125912. doi: 10.1371/journal.pone.0125912

Fig 8. MiR-183 cluster miRNAs coordinately target FOXO1 mRNA in bovine granulosa cells.

Fig 8

Putative miR-183 cluster binding sites and their genomic coordinates in the 3´-UTR of bovine FOXO1 mRNA were designed. Plasmid with wild-type and mutant sequences (underlined) for miRNA binding were fused downstream the firefly luciferase gene between SacI and XhoI restriction sites. The PGK promoter and transcription start site (TSS) of are indicated (A). Granulosa cells were transfected with or without bta-miR-183 mimic (B), with or without bta-miR-96 mimic (C), with or without bta-miR-182 mimic (D). The activity of luciferase was significantly inhibited when the bovine FOXO1 3´-UTR with wild type of miRNA binding sites was co-transfected with all miR-183 cluster miRNA mimics. However, the activity of Luciferase was not affected when FOXO1 3´-UTR with mutant sequences at the miRNA binding sites were co-transfected with or without miR-183 cluster miRNA mimics.