Table 1. Screening of a minilibrary of Brucella REC proteins.
| Prey | Bait | Input clones 1 | Positive clones 2 | Double positive clones 3 | Analyzed clones 4 |
|---|---|---|---|---|---|
| REC minilibrary | LOVHK | 39,800 (100) | >500 (1.3) | >500 | 20 (100% LovR) |
| PdhS-HK | 15,600 (100) | >500 (3.2) | >500 | 5 (100% DivK) | |
| Empty vector | 23,500 (100) | 23 (0.1) | ND | ND | |
| REC minilibrary (without LovR) | LOVHK | 57,000 (100) | >300 (0.52) | >60 | 10 (70% PhyR) |
| Empty vector | 51,000 (100) | 139 (0.27) | ND | ND |
1 Input clones were calculated from the number of colonies that grew in non-selective screening medium (see Materials and Methods for details).
2 Number of positive clones that grew in selective screening medium. The percentage of positive clones relative to total clones is indicated between parentheses.
3 Number of positive clones that grew in dual selective screening medium.
4 Number of positive clones analyzed by PCR and sequencing. RR identity and percentage is indicated between parentheses. ND: not determined.