Fig 7. Knockdown of TRAFD1 inhibits acidification of osteoclasts.

Acidification assay was performed with shTRAFD1 and control RAW264.7 cells. Cells were cultured on 24-well Osteo Assay plates for 5 days in the presence of RANKL (10 ng/ml) and stained with acridine orange. Representative confocal microscopy images are shown. White rectangles locate areas shown at higher magnification in insets, below. As a control for blocked acidification, some control cells were treated with bafilomycin A (200 nM; control + baf A). Scale bar = 50 μm.