Figure 3.

SILAC MS demonstrates that reconstituted YFP does not fall apart when BILN is removed. A, Schematic describing how labeled PSD95 retains complemented YFP after BILN washout despite NS3 protease cleavage. B, SILAC experimental design; the first sample is labeled with Lys*, Arg*, and BILN for 24 h and then immediately analyzed by MS, whereas the second sample undergoes an additional 24 h incubation in media lacking Lys*, Arg*, and BILN. C, PSD95-TS:YSOG2 amino acid sequence and peptides identified from MS analysis.