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. 2015 May 20;35(20):7736–7749. doi: 10.1523/JNEUROSCI.0004-15.2015

Figure 5.

Figure 5.

PKMζ-TS:YSOG2 is upregulated and localizes to synapses after forskolin and rolipram cLTP. A, PKMζ fusion to TS:YSOG2, before and after BILN addition and subsequent washout. B, Transfected neurons stimulated with forskolin and rolipram or left unstimulated, and untransfected neurons were fixed and immunolabeled for PSD95. PKMζ-TS:YSOG2 YFP fluorescence and PSD95 immunofluorescence were imaged as in Figure 4. Stimulated neurons show increased YFP fluorescence and PKMζ-TS:YSOG2 colocalization with PSD95. Scale bars: whole cell, 20 μm; magnified pseudocolor, 2 μm.