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. 2015 May 20;35(20):7736–7749. doi: 10.1523/JNEUROSCI.0004-15.2015

Figure 6.

Figure 6.

TS:YSOG3-PKMζ degrades rapidly in neuronal cell bodies and dendritic spines after forskolin and rolipram cLTP. A, Schematic indicating how neurons transfected with TS:YSOG3-PKMζ were treated with BILN for 24 h, then imaged once per hour for 10 h after BILN washout. B, Stimulated neuron YFP fluorescence decreased quickly over time, indicating that PKMζ is rapidly degraded. C, Unstimulated neuron YFP fluorescence decreased slowly in the 10 h following BILN washout, indicating that basal PKMζ remains more stable over time. D, YFP quantification in neuron cell bodies (n = 5 stimulated cell bodies; n = 5 unstimulated cell bodies). **p < 0.01 (unpaired two-tailed t test). Error bars indicate SEM. E, YFP quantification in dendritic spines (n = 53 stimulated dendritic spines; n = 51 unstimulated dendritic spines). ***p < 0.001 (unpaired two-tailed t test). Error bars indicate SEM. Scale bars, 20 μm.