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. 2015 May 20;35(20):7736–7749. doi: 10.1523/JNEUROSCI.0004-15.2015

Figure 8.

Figure 8.

PKMζ-TS:YSOG2 degrades more rapidly in neuronal cell bodies and dendritic spines after forskolin and rolipram cLTP. A, Schematic indicating how neurons transfected with PKMζ-TS:YSOG2 were treated with BILN for 24 h, then imaged once per hour for 10 h following BILN washout. B, YFP fluorescence in neurons stimulated with forskolin and rolipram decreases rapidly over time, indicating that PKMζ is rapidly degraded. C, Unstimulated neuron YFP fluorescence decreases slowly over time, indicating that PKMζ, produced at the basal level, is slowly degraded. D, YFP quantification in neuron cell bodies (n = 5 forskolin and rolipram stimulated cell bodies; n = 5 unstimulated cell bodies). Error bars indicate SEM. E, YFP quantification in dendritic spines (n = 54 forskolin and rolipram-stimulated dendritic spines; n = 55 unstimulated dendritic spines). ***p < 0.001 (unpaired two-tailed t test). Error bars indicate SEM. Scale bar, 20 μm.