Table 1.
Peptides identified from SILAC MS analysisa
| Sequence | Index (amino acids) | Mass (Da) | Ratio H/L |
RMSE | |
|---|---|---|---|---|---|
| BILN washout | No BILN washout | ||||
| VNDSILFVNEVDVR | 110–123 | 1617.836 | 0.10 | 7.8 | 0.5 |
| NASHEQAAIALK | 366–377 | 1251.657 | 0.07 | 12.8 | 3.0 |
| ANDDLLSEFPDK | 544–555 | 1362.630 | 0.06 | 4.8 | 0.6 |
| HCILDVSANAVR | 622–633 | 1353.662 | 0.02 | 4.4 | 1.1 |
| YIGSSGTGCVVIVGR | 923–937 | 1523.777 | 0.00 | 7.9 | 0.4 |
| IVLSGSGTSAPITAYAQQTR | 938–957 | 2020.059 | 0.00 | 52.9 | 0.8 |
| SLTPCTCGSSDLYLVTR | 1039–1055 | 1928.897 | 0.00 | 2.6 | 2.2 |
| AVDFIPVENLETTMR | 1112–1126 | 1733.866 | 0.00 | 8.1 | 0.7 |
| SPVFTDNSSPPAVTLTHPITK | 1127–1147 | 2208.143 | 0.00 | 11.3 | 0.6 |
| FLQGPETDQATVQK | 1313–1326 | 1560.778 | 0.02 | 9.3 | 5.7 |
aLys*- and Arg*-containing peptides are identified from the full PSD95-TS:YSOG2 right after a 24 h BILN pulse, although Lys*- and Arg*-containing peptides are identified from only the N- and C-termini of PSD95-TS:YSOG2 and not from the region excised by the NS3 protease when subjected to a 24 h BILN pulse and 24 h BILN washout. This indicates that the TS reporter is held together by complemented YFP despite NS3 protease cleavage upon BILN washout. RMSE, Root mean square error.