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. 2015 May 20;35(20):7878–7891. doi: 10.1523/JNEUROSCI.2179-14.2015

Figure 1.

Figure 1.

Pou4f3 expression is limited to hair cells. Expression of Pou4f3 was verified two ways: by labeling sectioned tissue with the Pou4f3 antibody and also using sectioned tissue from the Pou4f3-GFP reporter mouse. A, Pou4f3 (green) is selectively expressed in the nuclei of hair cells (red) from a mature (P56) WT mouse. An orthogonal view from the same section is shown in the top inset. The Pou4f3-GFP reporter mouse demonstrates expression in both inner and outer hair cells (lower inset). Scale bar, 100 μm. B, Pou4f3+/DTR mice were genetically engineered to contain the DT receptor downstream of the Pou4f3 promoter. Insertion of the DT receptor downstream of the Pou4f3 promoter creates a mouse model in which sensory hair cells in the inner ear can be selectively ablated after a systemic injection of DT.