Table 1.

Timeline of experiments and analysis

Pou4f3 expression
Tissue collected	P2, P5, P22, and P75
Analysis	Cochlea and brainstem tissue labeled for Pou4f3
Hair cell survival in vitro
Age harvested	P2–P3
Time in culture before treatment	24 h
Days of treatment before fixing	3
Analysis	Cultured cochlea labeled and imaged
Neonatal in vivo (cochlear, SGN, and CN changes)
Age at time of DT injection	P2 (4 ng/g) or P5 (5 ng/g)
Tissue collected	2, 4, 6–8, 10, 14, 21, and 70+ dpi
Analysis	Cochlea labeled and hair cells counted, Rosenthal's canal labeled and SGNs counted, cochlear nucleus labeled and neurons counted
Mature in vivo (ABR testing)
Age at time of DT injection	P28–P35 (25 ng/g)
Mice tested	Immediately before and 1, 3, 5, 8, and 21 dpi
Tissue collected	Immediately after ABR testing at each time point
Analysis	ABR threshold identified, cochlea labeled, and hair cells counted
Mature in vivo (dose response)
Age at time of DT injection	P30–P42 (5, 15, or 25 ng/g)
Tissue collected	8 and 21 dpi
Analysis	Cochlea labeled and hair cells counted
Mature in vivo (cochlear, SGN, and CN changes)
Age at time of DT injection	P21–P42 (25 ng/g)
Tissue collected	2, 4, 6–8, 10, 14, 21, and 70+ dpi
Analysis	Cochlea labeled and hair cells counted, Rosenthal's canal labeled and SGNs counted, cochlear nucleus labeled and neurons counted