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. 2015 May 20;35(20):8021–8034. doi: 10.1523/JNEUROSCI.0214-15.2015

Figure 1.

Figure 1.

EGFP expression in peripheral sensory neurons and primary sensory afferent fibers of Scn10a-EGFP mice. A, Schematic of linearized construct used to generate transgenic mouse line. A 3.7 kb sequence upstream of a 24 kb intron and transcriptional start site (TSS) of the Scn10a gene was used as the promoter to drive EGFP expression in transgenic mice. B–D, Top, Phase-contrast images. Bottom, EGFP fluorescence images. B, Thoracic DRG. C, Dorsal root branch. D, Dorsal side of a transverse spinal cord slice (300 μm). Note the EGFP fibers terminating in Lamina II of the spinal cord. E, Cross-section of mouse footpad (glabrous) skin. Top, Hematoxylin and eosin stain of glabrous skin section clearly labeling the stratum corneum (sc), epidermis (ep), and dermis (d) layers. Bottom, Fluorescence image of skin section taken before hematoxylin and eosin staining. Note the EGFP fibers extending into dermal papillae and terminating in the dermis layer, not crossing into the stratum spinosum layer of the epidermis. Scale bars, 100 μm.