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Correlation of EGFP expression with Scn10a transcript in DRG neurons from Scn10a-EGFP mice. A, Schematic of primer set used to amplify Scn10a transcript by single-cell PCR. Primers were designed to span 6 exons (exon 18–23) and generate a 742 bp amplicon. B, The resultant PCR products were separated and visualized on a 1.5% agarose gel. β-actin amplicon is 500 bp in length.
