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. 2015 May 20;35(20):8021–8034. doi: 10.1523/JNEUROSCI.0214-15.2015

Figure 5.

Figure 5.

Characterization of EGFP-expressing DRG neurons from Scn10a-EGFP mice. A, Growth factor dependence of EGFP expression in DRG neurons from transgenic mice under untreated control (left), GDNF (middle), or NGF (right) conditions after 6 d in culture (DIC). Top, Phase-contrast images. Bottom, EGFP fluorescence images. Scale bar, 50 μm. B, Phase-contrast (left), EGFP (middle), and red fluorescence (right) images of IB4-AlexaFluor-568-stained DRG neurons from transgenic mice. White arrowhead indicates EGFP-positive DRG neurons not bound to IB4. Scale bar, 50 μm. C, Distribution of EGFP-positive DRG neurons from Scn10a-EGFP mice (white bars). Purple-filled bars indicate EGFP-positive DRG neurons that were also IB4-positive. The IB4-positive population accounts for a large proportion of small- to medium-sized EGFP-positive neurons.