Figure 6.
Lack of EGFP expression in placodal-derived ganglia from Scn10a-EGFP mice. A, Nodose ganglia. Ai, Phase-contrast (top) and EGFP fluorescence (bottom) images of the same nodose ganglion. EGFP fluorescence was predominantly at the rostral end of the nodose ganglion. Arrow indicates the rostral (R)-caudal (C) orientation. Aii, Fluorescence image of the jugular-nodose ganglion complex from an Scn10a-EGFP mouse. JG, Jugular ganglion. Aiii, Immunohistochemistry of a sectioned nodose ganglion. Lower-magnification images are on the left; higher-magnification images are on the right. There is no EGFP fluorescence in the nodose section (top) but an abundance of Nav1.8-IR (bottom). B–D, Top, Bright-field images. Bottom, EGFP fluorescence images. B, Petrosal ganglion, indicated by blue arrow. C, Geniculate ganglion, indicated by blue arrow. D, Trigeminal ganglion, distal portion indicated by blue arrow. E, Summary of nuclear injection experiments with primary rat neurons and plasmid construct consisting of the putative Scn10a promoter and EGFP gene. Ei, Sample images of positive (above) or negative (below) neuron for Scn10a promoter-EGFP construct expression. Positive marker for successful nuclear injections, dsRed2nuc, shown on the right. Eii, The number of positive cells, expressed as a percentage of total neurons injected, and 95% confidence intervals are represented on graph. A total of 46 rat DRG neurons (from 4 different animals) and 103 rat nodose neurons (from 5 different animals) were injected. The proportion of injected neurons expressing EGFP was significantly different between both groups. ***p < 0.001 (Fisher's exact test). Unless otherwise stated: scale bar, 50 μm.