Table 1.
Characterization of the EGFP-positive peripheral sensory neuron populationa
| Label | % positive for label | % double-labeled cells (as % EGFP-positive cells) | % double-labeled cells (as % label-positive cells) |
|---|---|---|---|
| For DRG neurons | |||
| EGFP, transgene expression | 45.7 ± 2.2 (40) | ||
| EGFP-IR | 35.5 ± 3.6 (4) | 97.9 ± 1.2 | 90.7 ± 2.8 |
| Nav1.8 | 44.6 ± 2.2 (12) | 69.7 ± 1.6 | 80.2 ± 4.6 |
| IB4 | 35.4 ± 1.5 (18) | 56.7 ± 1.7 | 75.2 ± 4.2 |
| CGRP | 14.6 ± 1.1 (5) | 18.7 ± 0.9 | 61.4 ± 4.0 |
| Substance P | 8.0 ± 0.8 (4) | 7.2 ± 0.9 | 45.9 ± 4.7 |
| CtB | 36.5 ± 1.3 (12) | 9.6 ± 1.0 | 11.4 ± 1.7 |
| NF200 | 25.9 ± 1.1 (3) | 8.6 ± 1.4 | 10.8 ± 3.3 |
| TH | 9.0 ± 0.7 (5) | 5.6 ± 0.3 | 32.2 ± 3.9 |
| Nav1.7 | 91.3 ± 3.0 (9) | 95.8 ± 1.7 | 41.9 ± 5.0 |
| For ND neurons | |||
| EGFP, transgene expression | 7.0 ± 1.2 (13) | ||
| Nav1.8 | 46.1 ± 9.0 (5) | 82.9 ± 10.2 | 14.6 ± 3.8 |
aSummary of live-cell staining and ICC experiments on dissociated DRG or ND neurons isolated from Scn10a-EGFP mice. Fluorescence from individual neurons measured using a custom program written with Igor software (detailed in Materials and Methods). Staining with secondary antibody alone (i.e. no primary antibody) was used to determine the boundary between positive and negative red fluorescence intensity, and the boundary between positive and negative green fluorescence was set at 750 (for the two discrete populations of EGFP fluorescence intensity, see Fig. 3B). Double-labeled (EGFP-positive and label-positive) neurons expressed as either a percentage of total EGFP-positive (middle column) or label-positive (right column) neurons. n values for each group are biological replicates (different animals) and indicated in parentheses.