Table 1.

Primer sequences of target and reference genes.

Target gene	Product size (base pairs)	Annealing temperature (°C)	Sequence
Actb	200	66.5°C	Forward: 5′ CTCTGGCTCCTAGCACCATGAAGA 3′
		66.3°C	Reverse: 5′ GTAAAACGCAGCTCAGTAACAGTCCG 3′
Alpl	96	61.3°C	Forward: 5′ GGCCAGCTACACCACAACA 3′
		60.0°C	Reverse: 5′ CTGAGCGTTGGTGTTATATGTCTT 3′
Bglap	102	n.a.	Qiagen (QuantiTect Primer Assay KIT BGLAP)
			Cat. number: QT00259406
			(commercial product, no sequence available)
Fmod	145	62.7°C	Forward: 5′ AGCAGTCCACCTACTACGACC 3′
		62.2°C	Reverse: 5′ CAGTCGCATTCTTGGGGACA 3′
Hprt	173	67.1°C	Forward: 5′ GAGGAGTCCTGTTGATGTTGCCAG 3′
		66.4°C	Reverse: 5′ GGCTGGCCTATAGGCTCATAGTGC 3′
Runx2	207	60.3°C	Forward: 5′ CCAACCGAGTCATTTAAGGCT 3′
		60.8°C	Reverse: 5′ GCTCACGTCGCTCATCTTG 3′