TABLE 1.
Acceptor specificity of d-Rha-transferase WbpZa
| Compound (concn, mM)b | Activity (%) |
|---|---|
| GlcNAcα-PO3-PO3-(CH2)11-O-Ph (0.1) | 100 |
| GlcNAcα-PO3-PO3-AnthrU (0.1) | 57 |
| GlcNAcα-PO3-PO3-(CH2)9-CH3 (0.1) | 80 |
| GlcNAcα-O-CO-CH2-CO-O-(CH2)11-O-Ph (0.5) | <1 |
| GalNAcα-PO3-PO3-(CH2)11-O-Ph (0.1) | 94 |
| GalNAcα-PO3-PO3-AnthrU (0.1) | 47 |
| GalNAcα-p-nitrophenyl (1) | <1 |
| GalNAcα-Bn (1) | <1 |
| 4-Deoxy-GalNAcα-Bn (1) | <1 |
| 3,4-Dideoxy-GalNAcα-Bn (1) | <1 |
| 6-Deoxy-GalNAcα-Bn (1) | <1 |
| GlcNAcα-Bn (1) | <1 |
| GlcNAcβ-Bn (1) | <1 |
| N-Butyryl-glucosamineβ-S-2-naphthyl (1) | <1 |
| GlcNAc (1) | <1 |
| GalNAc (1) | <1 |
| d-Rha (1) | <1 |
| d-Rhaα-Bn (1) | <1 |
| A-(GalNAcα)T (1) | <1 |
| Acetyl-(GalNAcα)T-NH2 (1) | <1 |
| AHGVT-(GalNAcα)SAPDTRPAPGSTAPPA (1) | <1 |
a
Neutral compounds were assayed by the AG1x8 method, and sugar-diphosphate-lipids were assayed by the C18 Sep-Pak method (23). Radioactive product was quantified by scintillation counting. Anthracene-containing compounds were also separated after the incubation by HPLC and then estimated by their fluorescence intensity (24).
b
AnthrU, anthracenyl-undecyl.