TABLE 2.
NMR (600 MHz) parameters of d-Rha-transferase products d-Rhaα1-3GlcNAcα-PO3-PO3-(CH2)11-O-phenyl and d-Rhaα1-3GalNAcα-PO3-PO3-(CH2)11-O-phenyl
| Position | Rha-GalNAc-PP-PhU |
Rha-GlcNAc-PP-PhU, 1H ppm | |
|---|---|---|---|
| 1H ppm | 13C ppm | ||
| Rha-1 | 4.94 (s) | 96.3 | 5.05 |
| Rha-2 | 3.94 (o) | 70.0 | 3.95 |
| Rha-3 | 3.65 (o) | 70.5 | 3.62 |
| Rha-4 | 3.43 (o.t, J = 9.2Hz) | 71.7 | 3.33 |
| Rha-5 | 3.59 (o) | 69.1 | 3.51 |
| Rha-6-Me | 1.30 (o) | 16.8 | 1.20 (o) |
| GlcNAc-1 | 5.39 (m) | ||
| GlcNAc-2 | 3.99 (o) | ||
| GlcNAc-3 | 3.79 | ||
| GlcNAc-4 | 3.55 (o) | ||
| GalNAc-1 | 5.53 (dd, J = 6.7,3.8) | 94.7 | |
| GalNAc-2 | 4.39 (br, dt, J = ∼10.4,2.4) | 47.9 | |
| GalNAc-3 | 3.97 (dd, J = 11.1,2.8) | 72.5 | |
| GalNAc-4 | 4.23 (br, d, J = ∼2) | 64.4 | |
| GalNAc-5 | 4.15 (dd, J = 7.0,5.4) | 71.9 | |
| GalNAc-6 | 3.78, 3.72 (o) | 61.0 | |
| GalNAc-N-acetyl | 2.07 (s) | ||