TABLE 3.
Primers used in this study
| Primer | Sequence | Resulting construct |
|---|---|---|
| torC-L1 | CCGGTACCTGAAGCGATCTTAATGAGCAAATATG | pMR4, torR mutants, pMR32 |
| torC-U2 | CCGGATCCTCAACAATAACAATGTGATGTGGTGTCAGAGG | pMR4 |
| cat-LRed-U1 | ATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCGCTGGAGCTGCTTCGAA | MMR125, MMR130, MMR133 |
| cat-LRed-L1 | ATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATGAATATCCTCCTTAG | MMR125, MMR130, MMR133 |
| Psyn-U1 | CCTGACGGATGGCCTTTTTG | pMR19 |
| gfpNterm-L1 | CATCACCTTCACCCTCTCC | pMR19 |
| Tetafter-MCS-L1 | TATTATCGTGAGGATGCGTC | pMR19 |
| cfpNterm-U1 | CACTGGAGTTGTCCCAATTCTTG | pMR19 |
| Lac-pMR10-U1 | GGTGGCCGGAAGGCGAAGCGGCATGCATTTACGTTGACACCATCGAATGGGCAATCCAGTGCAAAGCTAG | MMR72 |
| Lac-pMR10-L1 | GCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGGAGCGATTGTGTAGGCTGGAG | MMR72 |
| torD-yfp-U1 | TAAGCCAGTTATTGCTGGTGTTAGTGGAGTGCGACCACCAAAACAGATAATAGAATTAAAGAGGAGAAAT | MMR141 |
| torD-yfp-L2 | TTAGTGGAGTGCGACCACCAAAACAGATAAAATTAATTCCGGGGATCCGT | MMR141 |
| Xyl-pKD13-U1 | TGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGGCAATCCAGTGCAAAGCTAG | MMR60, MMR72, MMR84, MMR141 |
| Xyl-pKD13-L1 | GTGATTTCAGCCCGGAGAAGCCACCGGATGTCGGTACGGCCAATTTCACTGAGCGATTGTGTAGGCTGGAG | MMR60, MMR72, MMR84, MMR141 |
| torR-SacI-L1 | CCCGAGCTCTTTATCAGCACACATCAGCG | torR mutants, pMR32 |
| torSTRdel-U1 | TACGGCAGAATATGAACAGATATGAACAGAATGAGTAAAACCCTCTGATGATTCCGGGGATCCGTCGACC | MMR133 |
| torSTRdel-L1 | AACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGTAGGCTGGAGCTGCTTC | MMR133 |
| D53A-FOR | GATTTAATTCTGCTGGCTATCAACTTACCCGA | pMR49, MMR140 |
| D53A-REV | TCGGGTAAGTTGATAGCCAGCAGAATTAAATC | D53A-TorR |
| torR-int-U1 | AGTTTCCGCATAATAACCCCTGTAGAATTATGGTTTAGTGAAGCGATCTTAATGAGCAAA | MMR138, MMR140 |
| torR-cat-rev | TAAGGAGGATATTCATATGGACCATGGGGCAACCAGCCCGGTGAG | MMR138, MMR140 |
| torR-cat-for | CTCACCGGGCTGGTTGCCCCATGGTCCATATGAATATCCTCCTTA | MMR138, MMR140 |
| torR-int-L1 | GCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCAGATTGCAGCATTACACGTC | MMR138, MMR140 |
| torS-BamHI-U1 | CCACACGGATCCTAATCCGCTTTGCGACTCAACCT | pMR29 |
| torS-HindIII-L1 | CCACACAAGCTTAACTGGCTGATATTGA | pMR29 |
| torT-upper-1 | CCCGGATCCCTAGCATAAAGCCTTATTATTG | pMR26 |
| torT-lower-1 | CCCGTCGACGATTTACTGGTGACGCAACATG | pMR26 |