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. 2015 May 18;197(12):2072–2082. doi: 10.1128/JB.02557-14

TABLE 2.

Fatty acid profiles of bacterial cells as determined by FAME GC-MS analysis

Characteristic and fatty acida Mean value for characteristicb
 % complementationc
OD600 of 1.5
 OD600 of 2.5
 OD600 of 4.5
 OD600 of 6.5
 OD600 of 1.5
 OD600 of 4.5
WT LIRI WT LIRI WT LIRI WT LIRI BHL OdDHL BHL + OdDHL BHL OdDHL BHL + OdDHL
% of total for individual or types of fatty acids
    12:0 0 0 0 0.1 0.1 0 0.4 0.2** NS NS NS 97**↓ 35**↓ 109**↓
    14:0 1.5 1.7** 1.5 1.8** 1.2 2.2** 2.3 3.5** NS NS 128*↓ 76**↓ 43**↓ 102**↓
    7Z-14:1 0.1 0.1 0 0 0 0 0.1 0.1** NS NS NS 84**↓ 53**↓ 92*↓
    9Z-14:1 0.2 0.1 0.2 0.2 0.2 0.1 0.6 0.5 NS NS NS NS NS NS
    15:0 0.2 0.3 0.3 0.3 0.2 0.2 0.2 0.2 NS NS −287*↑ NS 86*↑ 203*↑
    16:0 39 39 39 38.8 40.2 41.6** 38.3 41.7** NS NS NS NS NS 135*↓
    9Z-16:1 18 18.7** 18 19** 14.4 18.4** 10.1 15.7** NS NS NS 69**↓ 28**↓ 82**↓
    17:0 0.1 0.1 0.1 0.1** 0.1 0.1** 0.3 0.3 NS NS 19,353*↑ 46**↑ 29**↑ 126*↑
    9cyc-17:0 0.3 0.3 0.3 0.3 1.6 1** 3.5 2.1** 46**↑ NS 116*↑ 64**↑ 24**↑ 44**↑
    18:0 5.2 5.1 5.6 5.2 4.9 4** 14 10.7** NS NS 132**↑ 57**↑ NS 73*↑
    11Z-18:1 35.2 34.5** 34.6 34 35.6 31.9** 26.7 24.1** NS NS 172*↑ 62**↑ 34**↑ 111**↑
    11cyc-19:0 0.1 0.1** 0.1 0.1 1.2 0.4** 3.5 0.7** 2**↑ NS 112*↑ 54**↑ 16**↑ 52**↑
    13Z-22:1 0.1 0.1 0.1 0.1 0.1 0** 0.2 0.2 NS NS NS NS NS NS
    Sum SFA 46 46.2 46.6 46.3 46.8 48.1** 55.5 56.6 67*↓ NS 245*↓ 43**↓ 36**↓ 128**↓
    Sum CUFA 53.5 53.4 53 53.3 50.4 50.5 37.6 40.5** NS NS NS NS NS 314*↑
    Sum CPFA 0.5 0.3** 0.4 0.4 2.9 1.4** 7 2.8** 27**↑ NS 115*↑ 59**↑ 20**↑ 48**↑
ACCL 16.8 16.8** 16.8 16.7 16.8 16.7** 16.8 16.6** NS NS 166*↑ 62**↑ 32**↑ 101**↑
C18/C16 ratio
    All 0.7 0.7** 0.7 0.7 0.7 0.6** 0.9 0.6** NS NS 173**↑ 57**↑ 28**↑ 102**↑
    SFA 0.1 0.1 0.1 0.1 0.1 0.1** 0.4 0.3** NS NS 148**↑ 52**↑ NS 80*↑
    CUFA 2 1.8** 1.9 1.8** 2.5 1.7** 2.7 1.5** NS NS 144*↑ 60**↑ 26**↑ 90**↑
    CPFA 0.4 0.3** 0.4 0.3 0.8 0.4** 1 0.4** NS NS NS 56**↑ 16**↑ 80**↑
    CUFA + CPFA 1.9 1.8** 1.9 1.8** 2.3 1.7** 2.2 1.4** NS NS 145*↑ 61**↑ 27**↑ 95**↑
a

Individual fatty acids are indicated according to the carboxyl reference system; for example, “9Z-16:1” indicates a fatty acid with a 16-carbon acyl chain containing a cis C=C double bond at the ninth position from the carboxyl carbon (hexadec-9-enoic acid, or palmitoleic acid). Abbreviations: SFA, saturated fatty acid; CUFA, cis-unsaturated fatty acid; CPFA, cyclopropane fatty acid; ACCL, average carbon chain length.

b

Values for the wild type (WT) and the lasI rhlI mutant (LIRI) are shown. Values that are significantly different (P ≤ 0.01) from the WT value by two-tailed Student's t test are indicated (**).

c

Percent complementation was calculated as the percentage return toward the wild-type phenotype (values are shown only where P ≤ 0.05). Values that are significantly different from the value for the lasI rhlI mutant by two-tailed Student's t test are indicated by asterisks as follows: *, P ≤ 0.05; **, P ≤ 0.01. Values that are not significantly different (NS) are also indicated. Arrows pointing up and down indicate an increase or decrease in response to chemical complementation compared to the lasI rhlI mutant, respectively.