Fig. 2.

Western blot analysis for prolidase (a), α₂ integrin receptor (b), β₁ integrin receptor (c), IGF receptor (d), TGF-β1 (e), and NF-κB p65 (f) in control human skin fibroblasts (lane 1) and cultured in the medium containing 0.5 mM of enalapril (lane 2) or 0.5 mM of enalaprilat (lane 3). The mean values of six pooled cell homogenate extracts from six separate experiments are presented. The intensity of the bands was quantified by densitometric analysis. Densitometry was done with BioSpectrum Imaging System and presented as an arbitrary units. The same amount of supernatant protein (20 μg) was run in each lane. The expression of β-actin served as a control for protein loading (g)