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. 2015 May 20;9:117. doi: 10.3389/fnbeh.2015.00117

Figure 1.

Figure 1

Verification of antibody specificity. (A–C) Examples of western blots showing binding specificity of the antibodies used in the immunohistochemistry experiments: the GABA(A) antibody labeled a band of ca. 50 kDa (A) corresponding to the target receptor (Olsen and Tobin, 1990). Two bands were labeled by the GABA(B) receptor antibody (B), that correspond to the GABA(B) receptor subtypes 1 and 2 (Kaupmann et al., 1998). The GluN1 antibody (C) labeled a ca. 120 kDa band, corresponding to the reported kDa weight of GluN1 (Riou et al., 2012). β-actin (42 kDa) was used as a protein-loading control. Hippocampal tissue was used for assessment. Each lane corresponds to a separate sample. (D,E) Examples of immunoblots showing binding specificity of GluN2A (D) and GluN2B (E). The GluN2A and GluN2B antibodies labeled ca. 177 and ca. 178 kDa bands, respectively. These bands correspond to the reported kDa weights of the target receptors (Riou et al., 2012). Hippocampal tissue was used for assessment. Each lane corresponds to a separate sample.