Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| STRAINS | ||
| E. coli | ||
| BL21 (DE3) | F ompT hsdSB (rBmB) gal dcm (DE3) | Novagen |
| S. pneumoniae | ||
| NCTC7466 (D39) | S. pneumoniae reference strain | National Collection of Type Cultures (London, UK) |
| CMCC31109 | Serotype 1 | National Center for Medical Culture Collections (CMCC Beijing, China) |
| CMCC31203 | Serotype 3 | |
| CMCC31207 | Serotype 6B | |
| CMCC31446 | Serotype 4 | |
| CMCC31614 | Serotype 14 | |
| CMCC31689 | Serotype 19A | |
| CMCC31693 | Serotype 19F | |
| Clinical isolates (34) | ||
| Streptococcus pyogenes | Clinical isolates | Children's hospital, Chongqing, China |
| Streptococcus mitis | Clinical isolates | |
| Streptococcus mutans | Clinical isolates | |
| Streptococcus pseudopneumoniae | Clinical isolates | |
| S. aureus | ||
| ATCC29213 | S. aureus reference strain | American type culture collection (Maryland, USA) |
| Clinical isolates (3) | Children's hospital, Chongqing, China | |
| PLASMIDS | ||
| pEVP3 | Lee and Morrison, 1999 | |
| pEVP3-ftsW | Yan et al., 2013 | |