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Chromatin immunoprecipitation demonstrates that Setd8 occupies key regulatory elements in the Gata2 locus in uncultured fetal liver erythroblasts, including the −3.8 and +9.5 enhancers and the 1S and 1G promoters. The positive control is beta actin intron, and the negative control is a region upstream of the Gata2 locus with no known function. Data are presented as fold enrichment relative to total input control and represent the means and SEM from three independent experiments.
